FL → FL · about 128 miles
Orlando to Jacksonville bus & train schedule
Amtrak and FlixBus run 59 direct buses and trains a week from Orlando to Jacksonville. The fastest takes 2 hours 40 minutes; the first departure leaves at 4:45 AM and the last at 6:50 PM.
Timetable data from · Schedule last changed
- Buses and trains a week
- 59
- Fastest trip
- 2h 40m
- First departure
- 4:45 AM
- Last departure
- 6:50 PM
Timetable
Times are local to each stop. Timetables published by tds.ai, refreshed October 15, 2026. Schedules change, so check times when you book.
| Departs | Arrives | Duration | Days | Stops | Company |
|---|---|---|---|---|---|
| 4:45 AM | 7:45 AM | 3h 00m | Mon, Thu, Fri, Sat, Sun | Orlando Bus Station to Jacksonville Greyhound Bus Station | FlixBus Full timetable |
| 6:00 AM | 8:50 AM | 2h 50m | Daily | Orlando Bus Station to Jacksonville Greyhound Bus Station | FlixBus Full timetable |
| 11:45 AM | 2:25 PM | 2h 40m | Mon, Thu, Fri, Sun | Orlando Bus Station to Jacksonville Greyhound Bus Station | FlixBus Full timetable |
| 12:30 PM | 3:45 PM | 3h 15m | Daily except Tue | Orlando Bus Station to Jacksonville Greyhound Bus Station | FlixBus Full timetable |
| 12:36 PM | 3:51 PM | 3h 15m | Daily | Orlando to Jacksonville | Amtrak Full timetable |
| 12:45 PM | 5:10 PM | 4h 25m | Daily except Tue | Orlando Florida Mall Ave to Jacksonville Greyhound Bus Station | FlixBus Full timetable |
| 2:30 PM | 5:10 PM | 2h 40m | Daily | Orlando Bus Station to Jacksonville Greyhound Bus Station | FlixBus Full timetable |
| 3:40 PM | 6:20 PM | 2h 40m | Mon, Thu, Fri, Sat, Sun | Orlando Bus Station to Jacksonville Greyhound Bus Station | FlixBus Full timetable |
| 4:15 PM | 7:30 PM | 3h 15m | Mon, Thu, Fri, Sat, Sun | Orlando Bus Station to Jacksonville Greyhound Bus Station | FlixBus Full timetable |
| 6:50 PM | 10:13 PM | 3h 23m | Daily | Orlando to Jacksonville | Amtrak Full timetable |
Route map
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Where to board in Orlando Where you get off in Jacksonville The route of the busiest trip
Where to board in Orlando
Where buses and trains stop in Jacksonville
Return trip: Jacksonville to Orlando
Questions
How long is the bus or train from Orlando to Jacksonville?
The fastest direct bus or train takes 2 hours 40 minutes; the typical trip is 3 hours 15 minutes.
What time is the first departure from Orlando to Jacksonville?
The earliest departure is at 4:45 AM (Mon, Thu, Fri, Sat, Sun) and the last at 6:50 PM (Daily).
Which bus and rail companies go from Orlando to Jacksonville?
Amtrak and FlixBus run direct buses and trains on this route.
Where do I board in Orlando?
Buses and trains to Jacksonville leave from Orlando Amtrak station, Orlando Bus Station and Orlando Florida Mall Ave.