FL → FL · about 99 miles
Orlando to Gainesville bus schedule
FlixBus and Greyhound run 38 direct buses a week from Orlando to Gainesville. The fastest takes 1 hour 50 minutes; the first bus leaves at 5:15 AM and the last at 9:55 PM.
Timetable data from · Schedule last changed
- Buses a week
- 38
- Fastest trip
- 1h 50m
- First bus
- 5:15 AM
- Last bus
- 9:55 PM
Timetable
Times are local to each stop. Timetables published by tds.ai, refreshed October 15, 2026. Schedules change, so check times when you book.
| Departs | Arrives | Duration | Days | Stops | Bus company |
|---|---|---|---|---|---|
| 5:15 AM | 7:50 AM | 2h 35m | Daily | Orlando Bus Station to Gainesville Bus Stop | Greyhound Full timetable |
| 5:40 AM | 8:15 AM | 2h 35m | Daily | Orlando Bus Station to Gainesville Bus Stop | Greyhound Full timetable |
| 9:30 AM | 11:25 AM | 1h 55m | Daily | Orlando Bus Station to Gainesville | FlixBus Full timetable |
| 12:45 PM | 3:35 PM | 2h 50m | Daily except Tue | Orlando Florida Mall Ave to Gainesville | FlixBus Full timetable |
| 2:00 PM | 5:10 PM | 3h 10m | Daily except Thu | Orlando Florida Mall Ave to Gainesville | FlixBus Full timetable |
| 9:55 PM | 11:45 PM | 1h 50m | Mon, Thu, Fri, Sat, Sun | Orlando Bus Station to Gainesville | FlixBus Full timetable |
Route map
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Where to board in Orlando Where you get off in Gainesville The route of the busiest trip
Where to catch the bus in Orlando
Where the bus stops in Gainesville
Return trip: Gainesville to Orlando
Questions
How long is the bus from Orlando to Gainesville?
The fastest direct bus takes 1 hour 50 minutes; the typical trip is 2 hours 35 minutes.
What time is the first bus from Orlando to Gainesville?
The earliest departure is at 5:15 AM (Daily) and the last at 9:55 PM (Mon, Thu, Fri, Sat, Sun).
Which bus companies go from Orlando to Gainesville?
FlixBus and Greyhound run direct buses on this route.
Where do I catch the bus in Orlando?
Buses to Gainesville leave from Orlando Bus Station and Orlando Florida Mall Ave.